Orthogonality and Burdens of Heterologous AND Gate Gene Circuits in E. coli

Rapid engineering of versatile molecular logic gates using heterologous genetic transcriptional modules†

Rapid engineering of versatile molecular logic gates using heterologous genetic transcriptional modules† †Electronic supplementary information (ESI) available: Supplementary methods, gene regulatory sequences used, the characterised dose responses of the single-input Buffer gate and the three promoter inputs under various cognate chemical induction levels. See DOI: 10.1039/c4cc05264a Click here for additional data file.

We designed and constructed versatile modular genetic logic gates in bacterial cells. These function as digital logic 1-input Buffer gate, 2-input and 3-input AND gates with one inverted input and integrate multiple chemical input signals in customised logic manners. Such rapidly engineered devices serve to achieve increased sensing signal selectivity.

BIOTECHNOLOGICALLY RELEVANT ENZYMES AND PROTEINS Heterologous overproduction of β-fructofuranosidase from yeastXanthophyllomyces dendrorhous, an enzyme producing prebiotic sugars

The β-fructofuranosidase Xd-INV from the yeast Xanthophyllomyces dendrorhous is the largest microbial enzyme producing neo-fructooligosaccharides (neo-FOS) known to date. It mainly synthesizes neokestose and neonystose, oligosaccharides with potentially improved prebiotic properties. The Xd-INV gene comprises an open reading frame of 1995 bp, which encodes a 665-amino acid protein. Initial N-te...

Development of a modularized two-step (M2S) chromosome integration technique for integration of multiple transcription units in Saccharomyces cerevisiae

BACKGROUND Saccharomyces cerevisiae has already been used for heterologous production of fuel chemicals and valuable natural products. The establishment of complicated heterologous biosynthetic pathways in S. cerevisiae became the research focus of Synthetic Biology and Metabolic Engineering. Thus, simple and efficient genomic integration techniques of large number of transcription units are de...

The transduction channel TRPM5 is gated by intracellular calcium in taste cells.

Bitter, sweet, and umami tastants are detected by G-protein-coupled receptors that signal through a common second-messenger cascade involving gustducin, phospholipase C beta2, and the transient receptor potential M5 (TRPM5) ion channel. The mechanism by which phosphoinositide signaling activates TRPM5 has been studied in heterologous cell types with contradictory results. To resolve this issue ...